Validated Performance
>1,400
Experimental tests across multiple CAR architectures
0.06%
Detection sensitivity for CAR-positive cells
Ultra-low
Nonspecific background signal
Reproducible
Performance across independent batches
Overview
The SCOPE CD19 CAR Detection Reagent enables precise identification and quantification of CD19-directed CAR-expressing cells by flow cytometry.
- Antigen-based detection targeting the CAR binding domain
- Compatible across diverse CD19 CAR construct designs
- Optimized for low background and high signal intensity
Designed for reliable and reproducible CAR detection across standard laboratory workflows. Site-specific enzymatic labeling supports highly consistent reagent performance across production batches.
Key Benefits
High Specificity & Low Background
Designed for robust discrimination between CAR-positive and CAR-negative cells with minimal nonspecific binding.
Batch-to-Batch Reproducibility
Manufacturing processes optimized for consistent performance across independently produced reagent lots.
Workflow Integration
Compatible with standard flow cytometry staining protocols and standard instrument configurations.
Cross-CAR Compatibility
Validated across diverse CAR architectures, including CD8α hinge, IgG-derived hinge, CD28-based constructs, and minimal hinge formats (compact CAR designs with limited epitope accessibility).
Scientific Validation
Detection Sensitivity
Serial dilution experiments demonstrate reliable detection of rare CAR-positive populations down to 0.06%, with strong agreement between expected and measured values.
Detection of CAR-positive cells across serial dilution with consistent quantification at low frequencies.
Low Background Binding
The engineered antigen-based design minimizes nonspecific interactions, enabling clear separation between CAR-positive and CAR-negative populations with near-zero background signal.
Reproducible Performance
Independent reagent batches demonstrate highly consistent signal intensity and CAR-positive frequencies, supporting reliable quantitative analysis across experiments.
Why SCOPE CD19 CAR Detection Reagent
- Antigen-based detection for highly specific CAR recognition
- Reduced background compared to conventional Fc-based reagents
- Consistent performance across reagent batches
- Compatible with standard flow cytometry workflows
- Designed for reproducible and comparable results
Technical Highlights
Target
CD19-directed CAR constructs
Application
Flow cytometry
Formats
PE and Biotin
Kit size
30 tests
Use
Research use only (RUO)
Documents
Research Use Only (RUO): This product is intended for research use only and is not for use in diagnostic or therapeutic procedures.