Standard Operating Procedures

Protocols

Standard operating procedures for SCOPE detection reagents and reference materials. All protocols are optimized for reproducible flow cytometry results.

Last updated: February 2026

Reagents

  • SCOPE CAR Detection Reagent (3 µL per test)
  • Fluorochrome-conjugated backbone antibodies (e.g., CD45, CD3)
  • FACS staining buffer (any commercially available)
  • PBS (for final resuspension)

Materials

  • Suitable staining tubes (e.g., 5 mL polystyrene FACS tubes)
  • Centrifuge capable of 300 × g
  • Micropipettes and tips
  • Flow cytometer

Procedure

  1. 1Count cells and prepare 1 × 10⁶ PBMCs per test.
  2. 2Add 2 mL FACS staining buffer (any commercially available staining buffer), mix gently.
  3. 3Centrifuge cells at 300 × g for 5 minutes and discard supernatant.
  4. 4Resuspend the cell pellet in 100 µL FACS staining buffer.
  5. 5Add 3 µL SCOPE CAR Detection Reagent.
  6. 6Add fluorochrome-conjugated backbone antibodies as required (e.g., CD45, CD3).
  7. 7Mix gently by pipetting or brief vortexing.
  8. 8Incubate for 15 minutes at room temperature, protected from light.
  9. 9Add 2 mL FACS staining buffer, mix gently.
  10. 10Centrifuge at 300 × g for 5 minutes and discard supernatant.
  11. 11Repeat the wash once more with 2 mL FACS staining buffer and centrifuge again at 300 × g for 5 minutes.
  12. 12Discard supernatant and resuspend cells in 200 µL PBS.
  13. 13Proceed directly to flow cytometric acquisition and analysis.

Notes

  • All steps should be performed at room temperature unless otherwise specified.
  • Protect samples from light after adding fluorochrome-conjugated reagents.
  • Adjust antibody volumes according to manufacturer recommendations and titration results.
  • For Research Use Only (RUO).
For Research Use Only

SCOPE Reference Cell Mimics – Staining and Signal Transition

Protocol for using SCOPE reference cell mimics in instrument calibration and inter-laboratory standardization workflows.

Coming soon

Important Notes

  • All protocols are provided for research use only (RUO).
  • Protocols may be updated periodically. Check back for the latest versions.
  • For technical questions, contact info@blueray-precision.com